Short-term cocoa bioflavanol supplementation does not improve cold-induced vasodilation in young healthy adults.

Abstract

Cold-induced vasodilation (CIVD) is an oscillatory rise in blood flow to glabrous skin that occurs in cold-exposed extremities. Dietary flavanols increase bioavailable nitric oxide, a proposed mediator of CIVD through active vasodilation and/or withdrawal of sympathetic vascular smooth muscle tone. However, no studies have examined the effects of flavanol intake on extremity skin perfusion during cold exposure. We tested the hypothesis that acute and 8-day flavanol supplementation would augment CIVD during single-digit cold water immersion (CWI). Eleven healthy adults (24 ± 6years; 10M/1F) ingested cocoa flavanols (900mg/day) or caffeine- and theobromine-matched placebo for 8days in a double-blind, randomized, crossover design. On Days 1 and 8, CIVD was assessed 2h post-treatment. Subjects immersed their 3rd finger in warm water (42°C) for 15min before CWI (4°C) for 30min, during which nail bed and finger pad skin temperature were measured. Flavanol ingestion had no effect on CIVD frequency (Day 1, Flavanol: 3 ± 2 vs. Placebo: 3 ± 2; Day 8, Flavanol: 3 ± 2 vs. Placebo: 3 ± 1) or amplitude (Day 1, Flavanol: 4.3 ± 1.7 vs. Placebo: 4.9 ± 2.6°C; Day 8, Flavanol: 3.9 ± 1.9 vs. Placebo: 3.9 ± 2.0°C) in the finger pad following acute or 8-day supplementation (P > 0.05). Furthermore, average, nadir, and apex finger pad temperatures during CWI were not different between treatments on Days 1 or 8 of supplementation (P > 0.05). Similarly, no differences in CIVD parameters were observed in the nail bed following supplementation (P > 0.05). These data suggest that cocoa flavanol ingestion does not alter finger CIVD. Clinical Trial Registration Clinicaltrials.gov Identifier: NCT04359082. April 24, 2020.