Abstract
To observe the effects of metastasis-associated tumor gene family 2 (MTA2) depletion on human breast cancer cell proliferation and metastasis. A short-hairpin RNA targeting MTA2 was chemically synthesized and transfected into a lentivirus to construct Lv-shMTA2 for infection into the MDA-MB231 human breast cancer cell line. At 48 hours after infection cells were harvested and mRNA and protein levels of MTA2 were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. Cell viability and metastasis were assessed by CCK-8, wound-healing assay and Transwell assay, respectively. In addition, a xenograft model of human breast cancer was constructed to investigate cancerous cell growth and capacity for metastasis. After infection with Lv-shMTA2, mRNA and protein levels of MTA2 was significantly reduced (p<0.05) and MDA-MB231 cell proliferation and metastasis were inhibited (p<0.05). In addition, mean tumor size was smaller than that in control group nude mice (p<0.05) and numbers of metastatic deposits in lung were lower than in control group mice (p<0.05). Depletion of MTA2 affected MMP-2 and apoptosis-related protein expression. For the first time to our knowledge we showed that MTA2 depletion could significantly inhibit human breast cancer cell growth and metastasis, implying that MTA2 might be involved in the progression of breast cancer. The role of MTA2 in breast cancer growth and metastasis might be linked with regulation of matrix metalloproteinase and apoptosis.
Highlights
Breast cancer is a worldwide threat for female health
To address the question whether metastasis-associated tumor gene family 2 (MTA2) was critically involved in breast cancer cell proliferation and metastasis, we employed the RNA interference technique to knockout the expression of MTA2 in breast cancer cell line MDAMB231 cells
We found that mRNA level of MTA2 were significantly reduced when cells were infected with Lv-short-hairpin RNA (shRNA) targeting MTA2 (shMTA2) (p
Summary
Breast cancer is a worldwide threat for female health. Its incidence and mortality rates are increasing rapidly. Breast cancer is the fifth most common cause of cancer death worldwide, as it comprises of 10.4% of all cancer incidence making it the most common type of non-skin cancer in women. Worldwide 519,000 deaths were caused by breast cancer (7% of cancer deaths) in 2004 (Shaukat et al, 2013). Metastasis is a major concern for breast cancer prognosis (Shaukat et al, 2013), especially in developing countries where cancers are always diagnosed in the late stages (Wu et al, 2014). Better understanding of molecular mechanisms underlying breast cancer metastasis is still required
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
