Abstract

THE periodic appearance of auxospores in a uni-algal culture of the marine centric diatom Coscinodiscus concinnus W. Smith (Holmes and Reimann1) made possible an investigation of the temperature and light conditions under which auxospore formation occurs. By using the cross-gradient apparatus of Jitts et al.2 I was able to expose potential auxospore forming cultures to 64 different light and temperature combinations simultaneously. The cells were examined daily by removing the culture vessels from the gradient block and scanning the contents with an inverted microscope.

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