Short term survival of the related alive donor's renal implant and the compatibility HLA.

Abstract

Event Abstract Back to Event Short term survival of the related alive donor's renal implant and the compatibility HLA. Yolanda Trujillo1*, Elena Noris-Garcia1, Amparo Brito1, Nelioska Diaz1, Deisy Barberia1, Natacha Peña1, Rolando Vigueras1 and Sergio Arce1 1 Nephrology Intitute, Immunology, Cuba The separation of the lymphocytes T and B for magnetic pearls are a highly effective technique (compared with the technique of Fiber of Nylon) for the serological typing and the different modalities of tests crusades for the transplant. Objective: The magnetic pearls are covered with specific antibodies that recognize specific markers in the surface of the membrane of the lymphocytes T and B. When the lymphocytes have been separated, HLA typing is doing and later on the couple's donor-receiver selection. Results: To all they the protocol of the related alive donor's donating selection was applied settled down in the department of Immunology of the Institute of Nephrology: cross-match and auto reaction of lymphocytes to 4 °C, 22 °C and 37 °C, study of sensitization and the HLA typing class I and class II by means of the microlymphocitotoxicity test. The final cross-match carries out 48 hours before the transplant with the selected donor. It uses little quantity of blood, quick, a viability of a 98% of alive cells, without contamination and the concentration of cells required is obtained to carry out the HLA typing and other histocompatibility tests. Conclusion: With this technique we are able to introduce the typing HLA DR and HLA DQ for serology in the Donating couple's selection Lives Related in the Renal Transplant. Of the 100 studied receivers, 9 were identical HLA with their donor (siblings) with a survival of 100% and the rest HLA haploidentical (siblings and parents) with a survival of 96%. Keywords: histocompatibility tests, microlymphocitotoxicity test, HLA typing class I, HLA typing class II, cross-match test Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Trujillo Y, Noris-Garcia E, Brito A, Diaz N, Barberia D, Peña N, Vigueras R and Arce S (2013). Short term survival of the related alive donor's renal implant and the compatibility HLA.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00689 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Yolanda Trujillo, Nephrology Intitute, Immunology, Habana, Cuba, ytrujillo@infomed.sld.cu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Yolanda Trujillo Elena Noris-Garcia Amparo Brito Nelioska Diaz Deisy Barberia Natacha Peña Rolando Vigueras Sergio Arce Google Yolanda Trujillo Elena Noris-Garcia Amparo Brito Nelioska Diaz Deisy Barberia Natacha Peña Rolando Vigueras Sergio Arce Google Scholar Yolanda Trujillo Elena Noris-Garcia Amparo Brito Nelioska Diaz Deisy Barberia Natacha Peña Rolando Vigueras Sergio Arce PubMed Yolanda Trujillo Elena Noris-Garcia Amparo Brito Nelioska Diaz Deisy Barberia Natacha Peña Rolando Vigueras Sergio Arce Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.