Short-Term Simulated Warming Changes the Beta Diversity of Bacteria in Taiga Forests’ Permafrost by Altering the Composition of Dominant Bacterial Phyla

Abstract

Permafrost is widely degraded in the context of global warming. The spatial distribution of soil microbes in these cold habitats has received a lot of attention. However, knowledge on the changes in permafrost microbial communities following permafrost thaw is still limited. We used permafrost soil from a taiga forest for indoor experiments using pristine soil as a control (CK, −2 °C), simulating warming for 15 days at temperatures of 0 °C (T1), 2 °C (T2), and 4 °C (T3). Amplicons of the hypervariable V4 region of the bacterial 16S rRNA gene were sequenced to identify bacterial communities present in the soils of pristine and warming treatments. Warming increased the average relative abundance of Proteobacteria (5.71%) and decreased that of Actinobacteriota (7.82%). The Beta diversity changed (p = 0.001) and significantly correlated with the pH, microbial biomass carbon (MBC), and available potassium (AK) of the soil (p < 0.05). Warming further increased the Alpha diversity (Simpson index), changing the functional pathways of the bacterial communities, whereby secondary functional pathways produced significant correlations with bacterial phyla (p < 0.05). Combined, the results indicated that short-term warming altered the Beta diversity of soil bacteria in a taiga forest’s permafrost soil by decreasing the abundance of Actinobacteria and increasing that of Ascomycetes, while pH, MBC, and AK were identified as the soil factors influencing the structure and diversity of the bacterial communities.