Short Term Results of Fibrin Gel Obtained from Cord Blood Units: A Preliminary in Vitro Study.

Panagiotis Mallis,Effrosyni Panagouli,Manousos Makridakis,Eleni Georgiou,Catherine Stavropoulos-Giokas,Efstathios Michalopoulos,Jerome Zoidakis,Zetta Dimou,Vasiliki Gkioka,Ioanna Gontika,Antonia Vlahou

doi:10.3390/bioengineering6030066

Panagiotis Mallis, Effrosyni Panagouli + Show 9 more

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https://doi.org/10.3390/bioengineering6030066

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Abstract

Background: Recent findings have shown that the fibrin gel derived from cord blood units (CBUs) play a significant role in wound healing and tissue regeneration. The aim of this study was to standardize the fibrin gel production process in order to allow for its regular use. Methods: CBUs (n = 200) were assigned to 4 groups according to their initial volume. Then, a two-stage centrifugation protocol was applied in order to obtain platelet rich plasma (PRP). The concentration of platelets (PLTs), white blood cells (WBCs) and red blood cells (RBCs) were determined prior to and after the production process. In addition, targeted proteomic analysis using multiple reaction monitoring was performed. Finally, an appropriate volume of calcium gluconate was used in PRP for the production of fibrin gel. Results: The results of this study showed that high volume CBUs were characterized by greater recovery rates, concentration and number of PLTs compared to the low volume CBUs. Proteomic analysis revealed the presence of key proteins for regenerative medicine. Fibrin gel was successfully produced from CBUs of all groups. Conclusion: In this study, low volume CBUs could be an alternative source for the production of fibrin gel, which can be used in multiple regenerative medicine approaches.

Highlights

Fibrin gel, a platelet rich plasma (PRP) derivative, is mostly obtained from adult peripheral blood (APB) after a single blood collection [1,2,3]
The beneficial properties of fibrin gel in regenerative medicine are mostly attributed to the increased release of several growth factors—including transforming growth factor-β1 (TGF-β1), fibroblast growth factor (FGF), platelet derived growth factor A and B (PDGF AB and BB), vascular endothelial growth factor-A (VEGF-A) and hepatocyte growth factor (HGF)—through activation of platelets (PLTs) [9,10,11]
Fibrin gel is naturally derived hydrogel that can be developed in a few minutes and is characterized by unique viscoelastic properties

Summary

Introduction

A platelet rich plasma (PRP) derivative, is mostly obtained from adult peripheral blood (APB) after a single blood collection [1,2,3]. The beneficial properties of fibrin gel in regenerative medicine are mostly attributed to the increased release of several growth factors—including transforming growth factor-β1 (TGF-β1), fibroblast growth factor (FGF), platelet derived growth factor A and B (PDGF AB and BB), vascular endothelial growth factor-A (VEGF-A) and hepatocyte growth factor (HGF)—through activation of platelets (PLTs) [9,10,11]. These growth factors are contained in α-granules of PLTs, which are secreted during the blood coagulation cascade [12]. Conclusion: In this study, low volume CBUs could be an alternative source for the production of fibrin gel, which can be used in multiple regenerative medicine approaches

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