Short-term migration of transplanted Lin–Sca-1+c-kit+ hematopoietic stem cells after hippocampal ischemic injury of mice

Abstract

The study of migration and differentiation potential of different types of stem cells remains a problem for cell biology and regenerative medicine. The purpose of the study was to evaluate the ability of transplanted hematopoietic stem cells (HSC) of murine fetal liver to migrate into a zone of hippocampal ischemic injury at suboccipital intraventricular injection; and to assess their neural differentiation possibility in the early period after transplantation.Materials and methods. We modeled an ischemic injury of the hippocampus of FVB-wt mice and after 24 hours transplanted suboccipitaly fetal liver HSC of FVB-Cg-Tg(GFPU)5Nagy/J fetuses (transgenic by GFP). Sorting of Lin–Sca-1+c-kit+ HSC fractions was performed by FACS. After 7 and 14 days we performed immunohistochemical staining of brain slices for GFP, NeuN and GFAP markers.Results. On the 7th day after transplantation injected cells penetrated up to 100 µm from the wall of the 3rd ventricle, and on the 14th day single transplanted cells localized in the ischemic hippocampal CA1 region. Donor’s cells were round shape and did not express NeuN and GFAP markers. Features of reactive astrogliosis and neuronal death were kept in the hippocampal CA1 region of experimental animals, similar to the control group.Conclusion. Transplanted Lin–Sca-1+c-kit+ mice fetal liver HSC are able to survive and migrate to the area of hippocampal ischemic injury, but the possibility of their neuronal or astrocyte differentiation in 14-day time was not confirmed.