Short term ex vivo storage of kidneys cause progressive nuclear ploidy changes of renal tubular epitheliocytes.

Huaibin Sun,Wanhua Xian,Jun Tian,Xiangdong Yang,Tingting Xie

doi:10.1038/srep10341

Abstract

In renal transplantation, there has been considerable success, mainly in term of post-transplant graft function. However, upon closer scrutiny, it is known that severe dysfunction, including persistence of renal failure is seen after transplantation. The major condition that potentially cause significant lesion may be hypothesized to be related to the hypothermic approach to storage. To systematically examine these issues, we stored mammalian (sheep) kidneys in UWS at 4 °C for four different time points (0, 1, 3 and 6 hours). We obtained renal histological sections and examined tubular architecture as well as nuclear characteristics of tubular epitheliocytes. The results of our preliminary investigations suggest that there are temporal changes of tubular epitheliocytes, as well as genomic changes. These changes were also seen in tissues stored at room temperature. Our observations suggest the need for additional studies for redesigning of improvised storage solutions. Pilot studies using Celsior also revelaed similar kind of nuclear changes, suggesting that storage conditons are contributory, including perfusion versus static conditions. The results may explain persistence of tubular injury several days after orthotopic transplantation, and may potentially be contributory to delayed graft function (DGF).

Highlights

Activity) was demonstrated during the cold preservation
The results of our preliminary investigations suggest that there are temporal changes of tubular epitheliocytes, as well as genomic ploidy changes which may explain persistent of tubular injury several days after orthotopic transplantation, and may potentially be contributory to delayed graft function (DGF)
Celsior solution was used for storing kidneys in a small series of pilot experiments to establish whether the trend of changes seen in UWS storage conditions is observed in other storage solutions as well. 3 kidneys in each group were stored for 6 hours at both 4 and 25 °C

Summary

Introduction

Activity) was demonstrated during the cold preservation. Cold preservation decreased the total tubulin as well[18]. We stored freshly obtained high grade renal clear cell cancer biopsy samples and stored either in the cold or at room temperature to examine whether nuclear ploidy changes occurred in these cancer samples. The results of our preliminary investigations suggest that there are temporal changes of tubular epitheliocytes, as well as genomic ploidy changes which may explain persistent of tubular injury several days after orthotopic transplantation, and may potentially be contributory to delayed graft function (DGF). These changes may be dependent on the type of storage solution used for ex vivo storage, and provides a rationale for improvising current standards of storage solution. The time period we chose conform to the standard time periods currently used in protocols for storage of beating heart donor (BHD)-donated kidneys

Methods

Results

Conclusion