Abstract

BackgroundBioactivities of Docosahexaenoic acid (DHA) and Eicosapentaenoic acid (EPA) depend on their chemical forms. The present study was to investigate short term effects of triglyceride (TG), ethyl ester (EE), free fatty acid (FFA) and phospholipid (PL) forms of omega-3 fatty acid (FA) on lipid metabolism in mice, fed high fat or low fat diet.MethodMale Balb/c mice were fed with 0.7% different Omega-3 fatty acid formulation: DHA bound free fatty acid (DHA-FFA), DHA bound triglyceride (DHA-TG), DHA bound ethyl ester (DHA-EE) and DHA bound phospholipid (DHA-PL) for 1 week, with dietary fat levels at 5% and 22.5%. Serum and hepatic lipid concentrations were analyzed, as well as the fatty acid composition of liver and brain.ResultAt low fat level, serum total cholesterol (TC) level in mice fed diets with DHA-FFA, DHA-EE and DHA-PL were significantly lower than that in the control group (P < 0.05). Hepatic TG level decreased significantly in mice fed diets with DHA-TG (P < 0.05), DHA-EE (P < 0.05) and DHA-PL (P < 0.05), while TC level in liver was significantly lower in mice fed diets with TG and EE compared with the control group (P < 0.05). At high fat level, mice fed diets with DHA-EE and DHA-PL had significantly lower hepatic TC level compared with the control diet (P < 0.05). Hepatic PL concentration experienced a significant increase in mice fed the diet with PL at high fat level (P < 0.05). Furthermore, both at low and high fat levels, hepatic DHA level significantly increased and AA level significantly decreased in all forms of DHA groups (P < 0.05), compared to control groups at two different fat levels, respectively. Additionally, cerebral DHA level in mice fed diets with DHA-FFA, DHA-EE and DHA-PL significantly increased compared with the control at high fat level (P < 0.05), but no significant differences were observed among dietary treatments for mice fed diets with low fat level.ConclusionThe present study suggested that not only total dietary fat content but also the molecular forms of omega-3 fatty acids contributed to lipid metabolism in mice. DHA-PL showed effective bioactivity in decreasing hepatic and serum TC, TG levels and increasing omega-3 concentration in liver and brain.

Highlights

  • Omega-3 polyunsaturated fatty acids such as Docosahexaenoic acid (DHA) and Eicosapentaenoic acid (EPA) contained in fish oil have long been suggested effective bioactive substances [1]

  • The present study suggested that total dietary fat content and the molecular forms of omega-3 fatty acids contributed to lipid metabolism in mice

  • DHA and EPA in natural fish oil exist as triglycerides (TG) and phospholipids (PL), while they can be ingested in the form of purified ethyl esters (EE) or free fatty acids (FFA) [13]

Read more

Summary

Introduction

Omega-3 polyunsaturated fatty acids such as DHA and EPA contained in fish oil have long been suggested effective bioactive substances [1]. They have been proposed outstanding antithrombotic effects and efficient in prevention of cardiovascular diseases [2,3] through modulation of blood lipids [4] and lipoproteins [5]. DHA and EPA in natural fish oil exist as triglycerides (TG) and phospholipids (PL), while they can be ingested in the form of purified ethyl esters (EE) or free fatty acids (FFA) [13]. The present study was to investigate short term effects of triglyceride (TG), ethyl ester (EE), free fatty acid (FFA) and phospholipid (PL) forms of omega-3 fatty acid (FA) on lipid metabolism in mice, fed high fat or low fat diet

Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call