Short-term effect of nitrate or water stress on nitrate reduction and malate fermentation pathways in yellow lupine (Lupinus luteus) nodules

Abstract

Seven-week-old plants (symbiotic stage) of yellow lupine (Lupinus luteus L. cv. Ventus) were subjected for 8 days to 5 mM nitrate treatment or to drought stress to search for possible activation of bacteroidal nitrate and nitrite reductases. Both treatments affected activities of malate dehydrogenase and aspartate aminotransferase in nodule cytosol and therefore are presumed to impose O2-limitation to nodule metabolism. However, no significant symptoms of senescence of nodules were found. Both nitrate treatment and drought stress increased rhizobial nitrate and nitrite reductase activities in contrast to noted decrease of corresponding activities in nodule cytoplasm. Differential regulation supports the hypothesis that bacteroidal enzymes can act in dissimilatory mode when nodule respiration is limited due to environmental stresses.