Short-term depression at primary afferent synapses in rat substantia gelatinosa region.

Abstract

Short-term synaptic depression is a widespread and predominant mechanism underlying the process of neural information. To study the short-term depression at primary afferent synapses between Adelta fibers and substantia gelatinosa (SG) neurons in the spinal cord, transverse spinal cord slices with dorsal root attached were made from young rats. With whole-cell voltage-clamp method, Adelta-fiber elicited excitatory post-synaptic currents (EPSCs) were recorded from SG neurons visualized by infrared microscope. Using the normalized peak amplitudes of EPSCs, the existence of short-term depression was examined at all six stimulus frequencies ranging from 0.5 to 20 Hz. Both paired-pulse and steady-state depressions became greater with the increasing stimulus frequency. External calcium concentration could significantly affect the degrees of paired-pulse and steady-state depressions, with paired-pulse depression more affected. Application of NMDA receptor antagonist had no significant effect on this depression. These results indicated that short-term synaptic depression exists at primary afferent neurotransmission in spinal cord and results from the presynaptic reduction in the number of quanta of transmitter released by impulses.