Short tandem repeats recruit transcription factors to tune eukaryotic gene expression

Connor A Horton,Amr M Alexandari,Michael G Hayes,Julia M Schaepe,Emil Marklund,Nilay Shah,Arjun K Aditham,Avanti Shrikumar,Ariel Afek,William J Greenleaf,Raluca Gordan,Julia Zeitlinger,Anshul Kundaje,Polly M Fordyce

doi:10.1016/j.bpj.2021.11.1305

Short tandem repeats recruit transcription factors to tune eukaryotic gene expression

Connor A Horton, Amr M Alexandari + Show 12 more

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https://doi.org/10.1016/j.bpj.2021.11.1305

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Journal: Biophysical Journal	Publication Date: Feb 1, 2022
Citations: 3	License type: publisher-specific-oa

Affiliation: Stanford University, University of California, Berkeley, Uppsala University, Stowers Institute for Medical Research, Duke University, CZ Biohub

#Elements In Genomic DNA #Transcription Factors Pho4 + Show 8 more

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Abstract

Transcription factors (TFs) regulate gene expression via sequence-specific binding to regulatory elements in genomic DNA. Short tandem repeats (STRs) are enriched in these regulatory elements and have been widely associated with changes in gene expression, but the mechanism by which STRs modulate expression remains unknown. To address this gap, we leveraged the MITOMI microfluidic platform to measure equilibrium binding and kinetics of basic-helix-loop-helix TFs Pho4 and MAX to their consensus binding sites, surrounded by various random or repetitive sequences.

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