Abstract
We compared the organisms isolated from 30,210 pairs of blood culture bottles by using BacT/Alert system and the conventional system. Overall, 2,575 (8.5%) specimens were culture positive for pathogenic organisms. The sensitivity for detection of pathogenic organisms with the BACT/Alert system (85.6%, 2,203 of 2,575) was significantly higher than that with the conventional method (74.1%, 1,908 of 2,575; P < 0.0001). However, Burkholderia pseudomallei was isolated less often with the BacT/ALERT system (73.5%, 328 of 446) than with the conventional system (90.3%, 403 of 446; P < 0.0001). This finding suggests that use of the conventional culture method in conjunction with the BacT/Alert system may improve the isolation rate for B. pseudomallei in melioidosis-endemic areas.
Highlights
The Gram-negative bacillus Burkholderia pseudomallei is a Tier 1 select agent and the cause of melioidosis.[1]
In a retrospective study conducted during January 1, 2009– July 31, 2011 as part of routine patient care at Sappasithiprasong Hospital, a 1,000-bed tertiary-care hospital in northeastern Thailand, we compared the organisms isolated from more than 30,000 pairs of blood culture bottles by using the BacT/ Alert system and the conventional system
The culture medium is made in-house, blood culture bottles are incubated in a conventional incubator, and bacterial detection is made by direct visualization with or without regular sub-culture
Summary
The Gram-negative bacillus Burkholderia pseudomallei is a Tier 1 select agent and the cause of melioidosis.[1]. We compared the organisms isolated from 30,210 pairs of blood culture bottles by using BacT/Alert system and the conventional system. The sensitivity for detection of pathogenic organisms with the BACT/Alert system (85.6%, 2,203 of 2,575) was significantly higher than that with the conventional method (74.1%, 1,908 of 2,575; P < 0.0001).
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