Abstract
Specific thermal dehydration/rehydration of short polyethylene glycol (PEG) chains densely bound to nanotube channels was useful for aggregation suppression and refolding acceleration of proteins.
Highlights
Polyethylene glycols (PEGs) are very important materials in biological and medical applications involving proteins
We discovered that the interior short PEG chains in the so nanotube channels, in contrast to the corresponding free short PEG chain units in bulk solutions, suppressed protein aggregation and facilitated refolding of the denatured protein because of the unique thermal dehydration/rehydration ability of the interior PEG chains
Transmission electron microscopy (TEM) revealed that the binary self-assembly process produced nanotubes that were similar to 1-NTs with respect to their inner diameter and wall thickness
Summary
Polyethylene glycols (PEGs) are very important materials in biological and medical applications involving proteins. Lyophilized PEGn-NTs (1 1⁄4 7.0 mmol, glyPEGn 1⁄4 7.0 mmol, 2 1⁄4 7.0 mmol) were added to an aqueous solution of 1,8-ANS (35 mmol). The residual PEGn-NTs were washed several times with water to remove 1,8-ANS outside the nanotubes. UV-vis spectroscopic measurement a er complete destruction of the PEGn-NTs by heating in DMSO allowed us to calculate the amount (2.3–3.1 mmol) of the encapsulated 1,8-ANS. Aqueous dispersions of PEGn-NTs were dropped onto a carbon grid. Fluorescence microscopic observations of the PEGn-NTs encapsulating 1,8-ANS were carried out with an inverted microscope (Olympus IX71) equipped with a CCD camera (Hamamatsu ORCA-ER). The sample was prepared by dropping the aqueous dispersions of the PEGn-NTs encapsulating 1,8-ANS onto a glass slide. Fluorescence, UV-vis, and CD spectra were recorded with an F4500 spectrophotometer (Hitachi) equipped with a DCI temperature control unit (HAAKE), a U-3300 spectrophotometer (Hitachi) equipped with a BU150A temperature control unit (YAMATO), and a J-820 spectropolarimeter (JASCO) equipped with a PTC-423 L temperature control unit (JASCO), respectively
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