Short photoperiod attenuates CO2 fertilization effect on shoot biomass in Arabidopsis thaliana.

Abstract

The level of carbon dioxide (CO2) in the air can affect several traits in plants. Elevated atmospheric CO2 (eCO2) can enhance photosynthesis and increase plant productivity, including biomass, although there are inconsistencies regarding the effects of eCO2 on the plant growth response. The compounding effects of ambient environmental conditions such as light intensity, photoperiod, water availability, and soil nutrient composition can affect the extent to which eCO2 enhances plant productivity. This study aimed to investigate the growth response of Arabidopsis thaliana to eCO2 (800ppm) under short photoperiod (8/16h, light/dark cycle). Here, we report an attenuated fertilization effect of eCO2 on the shoot biomass of Arabidopsis plants grown under short photoperiod. The biomass of two-, three-, and four-week-old Arabidopsis plants was increased by 10%, 15%, and 28%, respectively, under eCO2 compared to the ambient CO2 (aCO2, 400ppm)i.e. control. However, the number of rosette leaves, rosette area, and shoot biomass were similar in mature plants under both CO2 conditions, despite 40% higher photosynthesis in eCO2 exposed plants. The levels of chlorophylls and carotenoids were similar in the fully expanded rosette leaves regardless of the level of CO2. In conclusion, CO2 enrichment moderately increased Arabidopsis shoot biomass at the juvenile stage, whereas the eCO2-induced increment in shoot biomass was not apparent in mature plants. A shorter day-length can limit the source-to-sink resource allocation in a plant inage-dependent manner, hence diminishing the eCO2 fertilization effect on the shoot biomass in Arabidopsis plants grown under short photoperiod.