Short-lived delayed luminescence of photosynthetic organisms I. Nanosecond afterglows in purple bacteria at low redox potentials

Abstract

A combined study of emissions of purple bacteria Rhodospirillum rubrum, Ectothiorhodospira shaposhnikovii and Thiocapsa roseopersicina was performed under conditions of low potential. It has been shown that a considerable part of the emission represents a delayed luminescence with a lifetime of about 5 ns and an activation energy ▵ E = 0.05 ± 0.03 eV). Intensity of this delayed luminescence is approximately equal to that of prompt fluorescence. It diminishes as temperature decreases and also as the intermediate acceptor I becomes reduced after prolonged illumination under low potential conditions. This luminescence represents a radiative decay of the intermediate state, P F, and the luminescence activation energy, ▵ E, reflects the energy barrier between P∗-890 and P F. The value of this barrier determined in the present work is much lower than those obtained previously [3,4,26] for the free-energy release during the primary act of charge separation, basing on redox potential techniques. The reason for this discrepancy is discussed. Delayed luminescence in the picosecond time range is predicted to exist under conditions of active photosynthesis as a result of a small (approx. 0.05 eV) energy barrier between P F and the excited singlet state of reaction center bacteriochlorophyll.