Abstract
The polymorphism in the gonadotropin-releasing hormone receptor (GnRHR) gene was detected by PCR single-strand conformational (PCR-SSCP) and DNA sequencing methods in 224 Boer goats. Two single nucleotide polymorphisms (SNPs), G891T and G757A, were found in the exon I of the goat GnRHR gene and their associations with litter size in Boer goats were evaluated. Association analysis showed that G891T and G757A had significant effects on litter size. In locus G891T, individuals with GT genotype had significantly larger litter sizes compared to GG genotype in the first and third parity. In locus G757A, ewes with GG genotype had significantly higher litter sizes than those of GA in the fourth parity. The present study primarily revealed an association between allele T at 891 locus of the GnRHR gene and high litter size in the first and third parity in Boer goats. These results provide evidence that the GnRHR gene may be significantly correlated with a high prolificacy in goats.
Highlights
Breeding Station, under common rearing conditions from 2003 to 2007
PCR products were electrophoretically detected on 1.2% agarose gel (5 V/cm) by staining with ethidium bromide
Denatured DNA was subjected to 10% polyacrylamide gel electrophoresis (PAGE) (39:1 acrilamyde:bisacrylamide) in 1 × tris-borate EDTA (TBE) buffer at constant voltage (120 V) for 10 16 h
Summary
Polymorphism of the GnRHR gene and its association with litter size in Boer goats Wucai Yang, Keqiong Tang, Chunyan Zhang, Deqing Xu2, Qunying Wen3 & Liguo Yang1#. Condition of use: The user may copy, distribute, transmit and adapt the work, but must recognise the authors and the South African Journal of Animal Science
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