Abstract
South African Angora goats are farmed under extensive production systems in relatively large herds. As a result, breeders make use of group and flock-mating systems that limit accurate parentage recording and selection efficiency. In this study the aim was to refine a panel of microsatellite markers suitable for parentage verification in South African Angora goats. The markers were first evaluated based on the number of alleles, allele frequency, PIC, HE, HO and individual exclusion probability, and secondly as part of a panel. Eighteen markers were tested in 192 South African Angora goats representing different family structures with known and unknown parent information. The final set of microsatellite markers, with the strongest exclusion and the least number of microsatellite markers, consisted of 14 microsatellite markers namely BM1258, BM1329, BM1818, BM7160, CSRD247, HSC, INRA63, INRABERN192, MCM527, OarFCB48, SRCRSP5, SRCRSP8, SRCRSP9 and SRCRSP24. This panel had a combined first-parent exclusion probability of 99.7% and it was possible to perform parental identification in a test family.
Highlights
South African Angora goats are farmed under extensive production systems in relatively large herds
South African Angora goats are primarily farmed under extensive production systems and herd sizes vary from 1000 to 2500 goats per herd
Mating systems used by breeders taking part in the National Small Stock Improvement Scheme primarily include group mating and flock mating, while commercial breeders make use of over-mating
Summary
Parentage verification of South African Angora goats, using microsatellite markers. Condition of use: The user may copy, distribute, transmit and adapt the work, but must recognise the authors and the South African Journal of Animal Science
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