Abstract
Faba bean is one of the most widely grown protein-producing food legumes. Faba bean plants showing symptoms of shoe stringed leaves, phyllody and flower abortion were observed in fields of Antequera (Málaga, Southern Spain). The etiology of this disease was investigated using graft transmission to periwinkle plants, polymerase chain reaction (PCR) with phytoplasma-specific primers, sequencing and phylogenetic analysis. We could not obtain amplification products from asymptomatic plants, whereas all tests were positive for phytoplasma infection from plants showing symptoms. Phylogenetic analysis indicated that this phytoplasma clustered in the 16SrIII group, the type member of which is X-disease phytoplasma. This is the first report of a phytoplasma infecting faba bean in Spain, and the first report of a phytoplasma in this group infecting faba bean.
Highlights
Faba bean is one of the most widely grown protein-producing food legumes
We report the detection of phytoplasma in faba bean plants by polymerase chain reaction (PCR) amplification and its characterization by sequencing and phylogenetic analysis
Fresh shoots from diseased faba bean plants were graft-transmitted to healthy periwinkle (Catharanthus roseus (L.) G
Summary
Faba bean is one of the most widely grown protein-producing food legumes. Faba bean plants showing symptoms of shoe stringed leaves, phyllody and flower abortion were observed in fields of Antequera (Málaga, Southern Spain). Primera detección de un fitoplasma infectando haba (Vicia faba L.) en España Palabras clave: legumbres, grupo del fitoplasma de la enfermedad X, secuencia rDNA 16S, reacción en cadena de la polimerasa, DNA de fitoplasma clonado, análisis filogenético. In Spain, faba bean production is concentrated in the Mediterranean region and, recently, new fields have been sown nearby to the food process industry.
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