Short communication: Effects of mammary biopsy in the dry period on activity and feeding behavior of dairy cows

Abstract

In dairy cattle, mammary biopsies are commonly used to study development and function of the mammary gland. The objective of this study was to investigate changes in activity and feeding patterns following the mammary biopsy procedure. Pregnant, nonlactating Holstein dairy cows (20 d before expected calving date) were exposed to either (1) a biopsy procedure, in which a mammary tissue sample (60 × 4 mm in diameter) was obtained from cows (n = 9) using a biopsy tool from the rear left quarter, following administration of a sedative (xylazine, 20 µg/kg of body weight) and local anesthetic (3 mL of lidocaine), or (2) a sham procedure, in which cows (n = 8) were removed from the pen and restrained for a similar duration of time as for the biopsy procedure. Behavior of cows was monitored for 5 d, beginning on the day following biopsy (approximately 14 h after the procedure). Cows were fitted with accelerometers to record daily lying time, lying bout frequency, and lying side. Daily individual feed intake was recorded using the Calan Broadbent feeding system, and feeding time and meal characteristics were determined from a subset of cows (n = 6 per treatment) using a change-of-state data logger to record the times the cows were accessing the feed bunk. Total daily lying time did not differ between treatments [13.9 h/d; standard error (SE) = 0.56], but biopsied cows had more frequent, shorter lying bouts on the biopsied side on d 1 following the procedure (6.67 vs. 4.25 bouts/d, SE = 1.03, and 70.0 vs. 97.0 min/bout, SE = 8.6; left vs. right side), whereas control cows had no side preference. We found no effects of treatment on feed intake and feeding time but, on the first day after treatment, biopsied cows had meals that were more frequent (7.2 vs. 4.6 meals/d; SE = 0.93) and tended to be shorter (28.2 vs. 60.9 min/meal; SE = 11.8) than control cows. In conclusion, we did not detect effects of mammary biopsy on feed intake or lying time during our time frame of observation, but activity patterns were altered, which could be indicative of increased overall restlessness and specific pain in the biopsied quarter.