Short Communication: Antibacterial activity of Cefquinome Sulfate in the presence of biofilm Staphylococcus spp isolated from bovine milk / Comunicação breve: Actividade antibacteriana do sulfato de Cefquinome na presença do biofilme Staphylococcus spp isolado do leite bovino

Abstract

Bovine mastitis is an inflammation of the mammary gland in dairy cattle usually caused by bacterial agents and cefquinome is a fourth-generation cephalosporin commonly used in treatment of this disease caused by Staphylococcus spp. in lactating cows due to a broad spectrum. On the other hand, biofilm formation provides protection for bacteria increasing the resistance to antibiotics and contributing to the persistence of infection. The objective of the present study was to assess if biofilm produced by Staphylococcus spp was able to protect the bacteria from cefquinome sulfate (CFQ) action for 24h exposure. Our findings showed that 95% (n=53/56) of Staphylococcus spp. were biofilm producer and the biofilm protected the most of species tested, except S. xylosus maybe due to the formation of a thin layer biofilm that did not interfere in the antimicrobial action. Thus, the effectiveness of cefquinome in persistent infections may be compromised due to a thicker biofilm.