Short Communication. An efficient axillary shoots propagate system for the living fossil plant - Wollemi pine (Wollemia nobilis)

Y Niu,X Pei,J Du,X Zhan,Q Zhang

doi:10.5424/fs/2013223-03997

Y Niu, X Pei + Show 3 more

Open Access

https://doi.org/10.5424/fs/2013223-03997

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Abstract

Aim of study: The aim of the work was to determine the optimum axillary shoot induction system by optimizing different factors.Area of study: The different factors which were pretreatment methods, media types, kinds of cytokinins and dark treatmentMaterial and methods: After pretreating and sterilizing, the stems of Wollemi pine which about 3cm long were cultured on three kinds of media (GD, DCR AND ms) with different concentration and type of cytokines (BA, ZT and TDZ), as well as dark treatment (0, and 4 weeks) and then exposure to tissue culture room. The experiments were randomized block designed and the results were analyzed with ANOVA.Main results: Rapid freezing in liquid nitrogen was the most effective pretreatment method, dark treatment for the first 4-weeks of stem explants cultured in induction media could increase the frequency of axillary shoot induction significantly. The highest frequency of axillary shoot induction (63%) was obtained when explants were cultured in 1/2GD medium with 6.8 μM TDZ combining 4-weeks dark treatment. Research highlights: The optimized conditions for shoot production in vitro can be useful for conservation in vitro of Wollemia nobilis.Keywords: Wollemia nobilis; in vitro conservation; tissue culture; an endangered relict conifer.

Highlights

Wollemi pine (Wollemia nobilis) was discovered in late 1994 as a small grove of trees in the Wollemi National Park, Australia
The highest frequency of axillary shoot induction (63%) was obtained when explants were cultured in 1/2GD medium with 6.8 μM TDZ combining 4-weeks dark treatment
The induction rate was subjected to the analysis of variance (ANOVA), and treatment means were compared by least significant difference (LSD) (Zhang and Chen, 2006)

Summary

Introduction

Wollemi pine (Wollemia nobilis) was discovered in late 1994 as a small grove of trees in the Wollemi National Park, Australia. It belongs to the ancient Araucariaceae family and represents a whole new monotypic genus (Jones et al, 1995). Wollemi pine is a curious Jurassic age relict, and an exciting new horticultural species. Most importantly, it could be served as a model adopted for the conservation of many other endangered horticultural species. The conservation method for this species has relied heavily on vegetative propagation

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