Short chain carboxylic acids production and dynamicity of microbial communities from co-digestion of swine manure and corn silage

Abstract

Short chain carboxylic acids (SCCAs) have attracted much attention due to their wide application and benefit of high economy. This study investigated the influence of organic load rates (OLRs) on short-chain carboxylic acids (SCCAs) production and microbial communities for co-digestion of swine manure (SM) and corn silage (CS) during four different OLRs stages in a semi-continuous mode. The results showed that relatively stable SCCAs concentration of 10.5–13.6 g COD/L and SCCAs yield of 0.42 mg COD/mg VS was achieved at OLR of 3.0 g VS/L·d. The maximum concentration of 19.1 g COD/L was achieved at 3.5 g VS/L·d. Volatile fatty acids (VFAs) (include acetic, n-butyric and caproic acids) accounted for approximately 80% of SCCAs. Hydrolysis bacteria (HB) including Clostridium, Terriporobacter, Intestinibacter, and Turiibacter decreased with the increase of OLR, while acidogenic bacteria (AB) including Acetobacter, Lactobacillus, Aeriscardovia, and Pseudomonas increased, resulting in insufficient degradation of CS.