Short- and long-term effects of lipopolysaccharide-induced inflammation on rabbit sperm quality

Abstract

Infections and resulting inflammation are widely known to cause transient or permanent male infertility. The objectives of this study were (1) to provide a suitable animal model of a sub-acute inflammatory state by intraperitoneally inoculating bacterial lipopolysaccharides (LPS) and (2) to define the short- and long-term effects of this state on the sperm quality of rabbit bucks. Two series of experiments were performed to accomplish these objectives. In experiment 1, 15 healthy New Zealand White rabbit bucks were divided into five homogeneous groups, receiving 25, 50, 100 and 150 μg/kg body weight (b.w.) of E. coli LPS dissolved in 2 ml of sterile saline or only saline (control), respectively. White blood cells (WBC), rectal temperature, feed intake and mating ability were observed for 3 consecutive days following inoculation. Inoculation of 50 μg/kg b.w. produces a reversible inflammation-like state that lasts for about 3 days, with minimal distress to the animals, and therefore it was used in our experiment. The major symptoms were fever and anorexia. Changes in WBC count and a moderate reduction in reproductive activity also occurred. In experiment 2, two groups of five rabbit bucks each were treated with 50 μg/kg b.w. E. coli LPS diluted in 2 ml of saline or only saline (controls), respectively. Semen samples were collected weekly up to 56 days after inoculation and the changes in semen characteristics were examined. During the first 3 days, semen volume and concentration decreased in both experimental groups, probably due to the high collection frequency. Sperm membrane integrity and the number of necrotic sperm were seriously affected 30 days after the LPS challenge, reaching a maximum at the end of the spermatogenic cycle (56 days). These results suggest that a sub-acute inflammation may cause infertility by compromising sperm membrane integrity which decreases a month after LPS-treatment. In addition, the rabbit could be a useful LPS animal model for further study of the effects of inflammation and the underlying mechanisms on sperm characteristics.