Shoot proliferation and callus regeneration from nodular buds of Drepanostachyum luodianense

Abstract

This research reports on an efficient shoot proliferation and callus regeneration system for bamboo. Young, semi-lignified branches with one lateral bud from Drepanostachyum luodianense (Yi et R. S. Wang) Keng f. were used as explants. Disinfection with 0.1% HgCl2 for 8 min was the optimum treatment and the best medium for bud initiation was Murashige and Skoog (MS) medium containing 3.0 mg L−1 6-benzyladenine (BA). Multiple shoots were induced from nodal shoot segments on MS medium containing 5.0 mg L−1 BA, 0.5 mg L−1 kinetin (Kin), and 1.0 mg L−1 naphthaleneacetic acid (NAA). The highest frequency of callus formation (65.6%) was on MS medium containing 4.0 mg L−1 2,4-dichlorophenoxyacetic acid (2, 4-D), 0.5 mg L−1 NAA, and 0.1 mg L−1 thidiazuron (TDZ). The optimum medium for callus proliferation was MS medium with 4 mg L−1 2,4-D, 0.5 mg L−1 TDZ and 0.5 mg L−1 NAA, and the optimum hormone combination was 4 mg L−1 BA + 0.5 mg L−1 NAA for callus redifferentiation (up to 85.6%). A 100% rooting was achieved on MS medium supplemented with 2.0 mg L−1 NAA and 0.5 mg L−1 3-indole butyric acid (IBA). Rooted plantlets were acclimatized in a greenhouse in humus soil + perlite (1:1) substrate. These micropropagated callus induction and regeneration systems for bamboo will be useful for genetic engineering and multiplication.