Abstract
The experiment was conducted in order to study the regeneration potential of cotyledon explants from some Iranian inbred lines of sunflower (Helian- thus annuus L.) and their Fj hybrids. Cotyledons of 2-day-old seedlings of three cytoplasmic male sterile inbred lines used as female (CMS 24, CMS 31 and CMS 60/52) and five inbred lines used as male (R-81.1, R-92, R-97, R-201 and R-232) and finally their 15 FI hybrids were plated on shoot induction medium, Murashige and Skoog (MS) containing 3% sucrose, 0.5% agar-agar and different concentrations of BAP and NAA. Analysis of variance showed significant differences for all organogenesis traits studied among parental genotypes and Fj hybrids, hormone combination treatments and genotype x hormone combination treatment interaction. Shoot organogenesis was optimized on MS medium with 1 mg I"1 BAP and 1 mg I"1 NAA. Interaction "(CMS 24 x R-201) x T2" showed the highest values for number of explants shooting per 100 explants plated (55%). Interaction "R- 92 x T2" showed the highest value for average number of shoots per explants plated (8.65). Interaction "R-81.1 x T2" showed the highest value for average number of shoots per explants shooting (23). Interaction "CMS 24 x T2" showed the highest value for number of explants rooting per 100 explants plated (35%).
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