Abstract
Fig, or Ficus carica, is a fruit tree from the Moraceae family and is widely grown in tropical and subtropical regions of the world. Fig plants are mainly propagated through grafting, air layering, and hardwood cutting whereby these methods were found to be less efficient. Plant tissue culture is efficient method to propagate plants, particularly to produce true-to-type platelets for mass multiplication. The aim of this study is to induce multiple shoot formation on Ficus carica cv. Japanese BTM 6 through identifying and optimising the concentrations of 6-Benzylaminopurine (BAP) and Zeatin suited for shoot formation. The axillary shoot tip explants were cultured in MS media supplemented with different concentrations of BAP and Zeatin (0, 0.5, 1.0, 1.5 and 2.0 mg/L) to determine the optimal concentration for the formation of multiple shoots. Number of shoots per explants and the differences in shoot height of explants were calculated after 8 and 12 weeks of culture respectively. Of all the treatments of BAP, MS media containing with 2 mg/L BAP marked the highest number of shoots per explant with the average value of 1.67 ± 0.33 while 1.5 and 2 mg/L of BAP produced the highest differences in shoot height with 0.51 ± 0.08 cm and 0.51 ± 0.07 cm after 12 weeks respectively. Murashige and Skoog (MS) media supplemented with 2 mg/L Zeatin showed the highest production of multiple shoots and differences in shoot height with the average of 0.83 ± 0.219 and 0.32 ± 0.04 cm respectively among all the different treatments of Zeatin. In this study, BAP performed better in shoot induction and elongation as compared to Zeatin for the cultivar Japanese BTM 6.
Highlights
The fig or Ficus carica is a fruiting tree of the Ficus genus from the family of Moraceae
The current study aims to establish an efficient protocol to for shoot formation of Ficus carica cv
Effect of BAP on Shoot Tip Explants of Ficus carica With reference to Fig. 1, shoots were induced from axillary shoot tips of Ficus carica cv
Summary
The fig or Ficus carica is a fruiting tree of the Ficus genus from the family of Moraceae. It is one of the earliest cultivated plant and is distributed throughout various regions including Mediterranean countries, Indian subcontinent, Far East, Latin America, and Southern California (Lansky & Paavilainen 2011). Dietary fiber and polyphenols are present at high amounts for both dried and fresh figs (Vinson 1999). The entire fig tree including the fruits, leaves, roots, latex and leafy branches have been utilised ailments for many types of illness which includes eye vision problems, colic treatment, indigestion and diarrhea (Barolo et al 2014). Previous study by Barolo et al (2014) reported the medicinal characteristics of the fig plant which included antioxidant properties, anti-helminthic, anti-fungal, and anti-carcinogenic, making it applicable in studies of ethno pharmacology
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