Ship‐in‐a‐Bottle Synthesis of Fluorescence‐labeled Nanoparticles: Applications in Cellular Imaging¶

Abstract

AbstractFluorescein (2‐(6‐hydroxy‐3‐oxo‐(3H)‐xanthen‐9‐yl) benzoic acid) has been prepared inside the pores of zeolite‐Y via ship‐in‐a‐bottle synthesis. Fluorescein, whose dimensions prevent it from entering through the ∼7 Å windows of the faujasite zeolite used, was prepared by the acid‐catalyzed reaction of resorcinol and phthalic anhydride. In this article we report initial spectroscopic data as well as an example of the usefulness of these fluorescence‐labeled nanoparticles for imaging applications such as confocal fluorescence microscopy. Encapsulated fluorescein shows a remarkable increase in photostability.