Abstract
We developed a web application ShinyR-DAM for analyzing Drosophila locomotor activity, sleep and circadian rhythms recorded by the Drosophila Activity Monitor (DAM) system (TriKinetics, Waltham, MA). Comparing with the existing programs for DAM data analysis, ShinyR-DAM greatly decreases the complexity and time required to analyze the data, producing informative and customizable plots, summary tables, and data files for statistical analysis. Our program has an intuitive graphical user interface that enables novice users to quickly perform complex analyses.
Highlights
We developed a web application ShinyR-DAM for analyzing Drosophila locomotor activity, sleep and circadian rhythms recorded by the Drosophila Activity Monitor (DAM) system (TriKinetics, Waltham, MA)
The Drosophila Activity Monitor (DAM) system, (TriKinetics, Waltham, MA), is a set of devices for recording Drosophila locomotor behavior that is widely used around the world
The DAM system records infrared beam breaks of flies walking in glass tubes
Summary
ShinyR-DAM accepts DAM system monitor files recorded by the TriKinetics DAMSystem[3] data acquisition software[14]. Users of the legacy DAMSystem[2] data acquisition software can convert their channel files into monitor files using our file converter https:// karolcichewicz.shinyapps.io/DAM2_to_DAM3_converter/. Using a graphical interface (Fig. 1), a user specifies the layout of the experimental conditions, including genotypes and treatments, and assigns them to specific monitors and channels. Each condition can have a user specified color for plot output. Each condition is assigned to a 32-channel DAM system monitor, but this setting can be overwritten to accommodate any experimental
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