Shiitake Mantar Ekstraktı İlaveli Yemlerin Gökkuşağı Alabalığında (Oncorhynchus mykiss) Büyüme, Non-Spesifik İmmun Parametreler ve in-vitro Aeromonas hyrophila Enfeksiyonuna Karşı Direnç Üzerine Etkileri

Abstract

The activity of Lentinula edodes (shiitake) medicinal mushroom extract were examined on the non-specific immune response and biometrical performance of rainbow trout (Oncorhynchus mykiss). Fish (20 g initial weight) were divided into 3 treatment groups (60 fish/group) and duplicated groups for 6 weeks having two experimental diets supplemented with 1-2% shiitake extract and a control diet. During the feeding process, immunological, biochemical, and biometrical observations were determined using the fish and blood samples taken at weeks 1, 2, 3, 4, 5, and 6, respectively. The results of immunological, biochemical, and biometrical parameters evaluation determined that the maximum influence occurs in rainbow trout fed with 2% shiitake extract. The amount of respiratory burst activity in the blood of fish in the trial groups significantly increased in each diet on the 2nd and 6th weeks compared to controls. The amount of total protein, bactericidal activity were significantly increased in fish being fed a mushroom supplemented diet. Cholesterol level decreased in fish blood, which fed with 2% shiitake extract supplemented diet at 3rd and 6th weeks. These results support the findings that the non-specific immune responses of rainbow trout was stimulated in fish by feeding shiitake medicinal mushroom extract yielding positive results in measured parameters compared to the control group also enhancing the overall growth performance of rainbow trout.