Abstract
Abstract Shielded Hydrophobic Phase(SHP) for Direct Sample Analysis of phenobarbital in serum was investigated for Therapeutic Drug Monitoring by studying the column stability and utilization parameters, and by comparison to the established fluorescence polarization immunoassays. This novel packing consisted of 5 um spherical silica particles with 100 A pores, bonded with polar and non-polar functionalities to the inside and outside of the particles. Proteins may be eluted, unretained with solvent front peaks while the drug/metabolites would undergo hydrophobic interaction, eluting later. For Direct Sample Analysis of phenobarbital in serum, 10 uL aliquots, after centrifuged at 9,500 × g for 20 minutes, were injected into the SHP guard column and column, and eluted with phosphate/ACN(9:1). Phenobarbital eluted with k′ of 3.4 at 3.8 minutes. Calibration was linear between 5 to 80 mg/L. Precision studies showed acceptable within-run and day-to-day coefficients of variation. Comparison with FPIA showed accep...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
