Shepherd's Purse Polyphenols Exert Its Anti-Inflammatory and Antioxidative Effects Associated with Suppressing MAPK and NF-κB Pathways and Heme Oxygenase-1 Activation.

Jinming Peng,Baohui Cheng,Jin Li,Kaikai Li,Kerui Zhu,Tianyong Hu,Jing Du

doi:10.1155/2019/7202695

Abstract

Shepherd's purse (Capsella bursa-pastoris (L.) Medik.), a wild herb as a traditional herbal medicine, has been proved with multiple healthy benefits. In this study, the chemical constituents of shepherd's purse were identified by UPLC-QTOF-MS/MS. The antioxidative and anti-inflammatory potential of shepherd's purse extract (SPE) were also investigated applying lipopolysaccharide- (LPS-) induced inflammation in RAW 264.7 macrophages and a carrageenan-induced mice paw edema model. Twenty-four chemical compounds were identified mainly including phenolic acids and flavonoids. The data also indicated SPE inhibited the productions of NO, PGE2, TNF-α, and IL-6 stimulated with LPS. In addition, SPE inhibited the increase of reactive oxygen species (ROS) and upregulated the expression of heme oxygenase-1 (HO-1). We further found that SPE inhibited the phosphorylation of P38 MAPK and activation of NF-κB. In vivo mice model also indicated that SPE showed strong antioxidative and anti-inflammatory activity.

Highlights

Inflammation which existed in obesity, elder bodies, is accompany with many diverse chronic diseases, such as insulin resistance, type 2 diabetes, vascular disease, chronic renal failure, several cancers, endocrine [1,2,3]
The functional foods have received increasing attention worldwide. It can supply the nutrients and provide many phytochemicals which play as a functional factor for human health, especially for these people with chronic disease or in the state of subhealth, such as chronic low-grade inflammation; these functional foods may be the best choice to improve their healthy rather than treatment
The results indicated that the production of TNF-α and IL-6 were significantly inhibited with the treatment of s purse extract (SPE) from 4 h

Summary

Introduction

Inflammation which existed in obesity, elder bodies, is accompany with many diverse chronic diseases, such as insulin resistance, type 2 diabetes, vascular disease, chronic renal failure, several cancers, endocrine [1,2,3] To counteract this chronic inflammatory status, nonsteroidal anti-inflammatory drugs (NSAIDs) were usually proposed as a treatment strategy [4]. The functional foods, for instance, some edible wild herbs, which with special health benefits, unique flavor, and with high nutritional values, may be a good choice for improvement of the chronic low-grade inflammation and its related diseases. These functional foods showed higher biosafety and can be and well accepted. Pharmacological studies proved that shepherd’s purse with various bioactivities, including

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