Abstract
Background Shen-Shuai-Ling Formulation (SSLF) has apparent effects on improving renal function, delaying the progression of chronic kidney disease (CKD). Methods Fifty male SD rats were randomly divided into 5 groups: Sham group, Model group, SSLF group, CPN group, and C + S group. The morphological changes and the collagen fibers of the rat kidneys were observed by HE staining. The expression of α-SMA, Col I, SHH, Gli1, and snail1 was detected by Western blot and qPCR. Then, the cells were divided into the control group, SHH group, and SHH + SSLF serum group. Results Compared with the Model group, the fibrosis in SSLF, CPN, and C + S groups was significantly alleviated. And, compared with those in the Model group, the expression of α-SMA, Col I, SHH, Gli1, Snail in SSLF, CPN, and C + S groups decreased remarkably. Conclusions SSLF remarkably improves renal function and alleviates renal interstitial fibrosis in UUO rats.
Highlights
chronic kidney disease (CKD) has become a prominent public health issue in the world. e global prevalence of CKD in 2017 was 9.1% (697.5 million cases)
It is an effective indicator of the disease prognosis. erefore, it is of great significance to study the mechanism of renal interstitial fibrosis (RIF) in order to delay the pathological process of CKD and preserve renal function
Fifty clean-grade male SD rats were randomly divided into five groups with 10 rats in each group: Sham operation (Sham) group, Model group, Shen-Shuai-Ling Formulation (SSLF) group, CPN group, and S + C group. e Sham group were first sedated with 5% sodium pentobarbital
Summary
CKD has become a prominent public health issue in the world. e global prevalence of CKD in 2017 was 9.1% (697.5 million cases). It has been confirmed that the severity of RIF is closely linked to the degree of renal function declination. It is an effective indicator of the disease prognosis. Shen-Shuai-Ling Formulation (SSLF) has apparent effects on improving renal function, delaying the progression of chronic kidney disease (CKD). Compared with the Model group, the fibrosis in SSLF, CPN, and C + S groups was significantly alleviated Compared with those in the Model group, the expression of α-SMA, Col I, SHH, Gli, Snail in SSLF, CPN, and C + S groups decreased remarkably. SSLF remarkably improves renal function and alleviates renal interstitial fibrosis in UUO rats
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