Sheet-like Janus hemostatic dressings with synergistic effects of cardanol hemostasis and quaternary ammonium salt antibacterial action.

Abstract

It is of utmost importance that bleeding should be stopped and infection be prevented in people with trauma. In this study, an anisotropic Janus mesoporous silica nanosheet (MSNS) with different functional groups was designed and prepared. In order to endow both sides of the MSNS with independent fast hemostasis and effective antibacterial action, the MSNS was modified with cardanol (CA) and 2,3-epoxypropyltrimethylammonium (GTA). The addition of CA significantly improved the hemostatic property of the MSNS. In a SD rat femoral artery injury model, the hemostatic time of CA-MSNS-GTA was 47% shorter than that of the MSNS, attributed to the sealing of the hydrophobic alkyl side chain and the adhesion of phenolic hydroxyl groups in CA. CA-MSNS-GTA could form a three-dimensional network with fibrin to further accelerate the coagulation process. This Janus material exhibited excellent antibacterial effects (∼90%) against Gram-positive bacteria (S. pneumoniae) and Gram-negative bacteria (E. coli) due to the presence of GTA. The cytotoxicity test showed that CA-MSNS-GTA exhibited biosafety, which provided safety guarantee for clinical applications in the future. This Janus dressing with different functions on two opposite sides provides synergetic multifunctional effects during wound healing.