Sheet beam x-ray fluorescence computed tomography (XFCT) imaging of gold nanoparticles.

Abstract

X-ray fluorescence computed tomography (XFCT) experiments have typically used pencil beams for data acquisition, which yielded good quality images of gold nanoparticles (AuNP) but prolonged the imaging time. Here we propose three novel collimator geometries for use with faster sheet beam XFCT data acquisition. The feasibility of a multipinhole, parallel, and converging collimator was investigated in a Monte Carlo study. A cylindrical water phantom with 2cm in diameter and 3cm in height containing 0.5-2mm diameter vials with 0.4%-1.6% AuNP concentrations was modelled by FLUKA. A 15 and 81keV monoenergetic x-ray sheet beam of 0.4mm in width was used to image the phantom with L-shell and K-shell XFCT, respectively, with a dose of 30mGy. The collimator thickness for L-shell and K-shell data acquisition was 3.3 and 5.1mm, respectively. The XFCT images resulting from three collimator geometries were generated using the maximum likelihood expectation maximization (MLEM) iterative reconstruction method. With a resolution of 0.4mm they were corrected for x-ray attenuation. The sheet beam XFCT images were compared against pencil beam geometry images that were generated using 55 translations. To assess image quality, the contrast-to-noise ratio (CNR) was evaluated for each vial. The Rose criterion was used to determine the lowest AuNP concentration detectable for each image. Among the three collimator geometry types, the sheet beam L-shell and K-shell parallel collimator XFCT images yielded AuNP sensitivity limits at 0.09% and 0.08%, respectively, for a 2mm diameter vial. The AuNP sensitivity limits of the pencil beam XFCT images were 0.07% and 0.01% for L-shell and K-shell XFCT, respectively. The L-shell parallel collimator AuNP imaging sensitivity approached that of the pencil beam geometry with a 55-fold reduction in imaging time. The AuNP sensitivity limits for the 1 mm diameter vial for the L-shell and K-shell parallel collimator XFCT images were 0.19% and 0.16%, respectively, and those of the pencil beam XFCT images were 0.08% and 0.01% for L-shell and K-shell XFCT, respectively. The remaining two collimator geometries resulted in a lower CNR and poorer image quality. For a 2mm diameter vial, the AuNP sensitivity limits for the L-shell and K-shell multipinhole collimator XFCT images were 0.23% and 0.52%, respectively, while for the L-shell and K-shell converging collimator XFCT images the AuNP sensitivity limits were 0.38% and 0.13%, respectively. This work demonstrates the feasibility of sheet beam L-shell XFCT imaging for small animal studies using parallel-oriented lead collimators which can detect AuNP concentrations approaching the level of pencil beam images with reduced imaging time.