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Abstract
When normal native or heparinised blood is forced under pressure through a filter with capillary sized holes, platelets aggregate during their passage through the filter. The effluent blood is collected from 0-3s (first phase) and between 10-20s (second phase); the platelets are counted, and the percentage retained is calculated. Aggregation in the first phase requires high shear, vWf and GP IIb/IIIa only, while divalent cations are essential for the second
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