Abstract
Effective visualisation of quantitative microscopy data is crucial for interpreting and discovering new patterns from complex bioimage data. Existing visualisation approaches, such as bar charts, scatter plots and heat maps, do not accommodate the complexity of visual information present in microscopy data. Here we develop ShapoGraphy, a first of its kind method accompanied by an interactive web-based application for creating customisable quantitative pictorial representations to facilitate the understanding and analysis of image datasets (www.shapography.com). ShapoGraphy enables the user to create a structure of interest as a set of shapes. Each shape can encode different variables that are mapped to the shape dimensions, colours, symbols, or outline. We illustrate the utility of ShapoGraphy using various image data, including high dimensional multiplexed data. Our results show that ShapoGraphy allows a better understanding of cellular phenotypes and relationships between variables. In conclusion, ShapoGraphy supports scientific discovery and communication by providing a rich vocabulary to create engaging and intuitive representations of diverse data types.
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