Abstract
We have designed and synthesized a peptide, WLFLLKKK (peptide-1), which has positive charges and a segment partitioned into the electrically neutral lipid membrane interface, and investigated its effect on the stability of the phosphatidylcholine (PC) membrane. Spacing of multilamellar vesicles of palmitoyloleoyl-PC increased greatly with an increase in peptide-1 concentration. The addition of 5 μM peptide-1 through a micropipet near the giant unilamellar vesicle (GUV) of dioleoylphosphatidylcholine induced several kinds of shape changes; for example, a discocyte was changed to two spheres connected by a neck, and small vesicles were budded into the outside of the spherical GUV. These results indicate that the de novo designed peptide-1 can be partitioned into the PC membrane interface and have a large effect on its structure and properties.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
