Abstract
An ecofriendly, specific, accurate and precise SFC-MS method (using TurboIonSpray probes) is described for the determination of ethambutol in its pharmaceutical dosage form as well as in human urine samples by using supercritical carbon dioxide (SC-CO2, 90%) as the mobile phase at a flow rate of 2 mL min−1 and methanol : formic acid (100 : 0.1 v/v, 10%) as a modifier at a flow rate of 0.2 mL min−1. Supercritical carbon dioxide (SC-CO2) is an environmentally friendly mobile phase which has outstanding penetration power, negligible toxicity and low viscosity. EMB was determined in less than 5 min using a C18 reverse-phase fused-core column (Inertsil ODS-C18, 100 mm × 4.6 mm, 5 μm). The method was validated according to the International Conference on Harmonization (ICH) and US-FDA guidelines with values of selectivity, specificity, linearity, extraction recovery, matrix effect, accuracy and precision in the working range of 0.1 to 20 μg mL−1 with an accuracy (% RE) of EMB of −6.00 to 6.00%. The proposed method was successfully applied to human urine samples and marketed formulations from various manufacturers.
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