Abstract
KUMAR1 reported the results of an investigation which seemed to demonstrate, for the first time, genetic recombination in the blue–green alga, Anacystis nidulans. This conclusion was based on an experiment in which a streptomycin-resistant and a penicillin-resistant strain were cultured together in the absence of antibiotics. Subculture of this mixture into liquid medium containing both antibiotics resulted in the growth of an apparently doubly resistant, recombinant strain. Because resistant clones had not been isolated, these results did not unequivocably demonstrate genetic recombination. Pikalek2 repeated Kumar's experiments and contested his conclusions by showing that the medium in which the apparently recombinant cells were growing lacked penicillin. He concluded that the observed growth was due to non-metabolizing streptomycin-resistant cells which were able to survive in penicillin for the short time before the decay of this antibiotic.
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