Abstract
Sexual isolation between the closely related species, D. persimilis and D. pseudoobscura, was studied by means of male multiple choice tests, female multiple choice tests, and multiple choice experiments involving the males and females of both species. The female choice and multiple choice experiments gave essentially the same result, a much higher degree of sexual isolation (I = 0.069 and I = 0.058), than the male choice experiments (I = 0.457). These results indicate that the high degree of sexual isolation between these two species is primarily due to the behavior of the females.
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