Abstract
Transformation of Gaeumannomyces graminis, a homothallic ascomycete, to benomyl resistance (BenR) or phleomycin resistance (PhleoR) enabled us to isolate crossed perithecia and analyze phenotypic and genotypic segregation of the selectable markers in ascospore progeny. One BenR transformant required nicotinic acid for growth and the relationship between the auxotrophy and BenR was examined. Normal distributions of both benomyl resistance and phleomycin resistance were observed in ascospore progeny from crosses between the auxotroph and a wild-type strain or between the auxotroph and a PhleoR transformant. Benomyl resistance always segregated with nicotinic acid auxotrophy in the progeny, indicating that the two genes encoding these traits were closely linked. A simple method for faster production of G. graminis perithecia is also described.
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