Abstract
BackgroundConogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time.ResultsTotally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues.ConclusionsOur study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be supportive for pest management studies in future.
Highlights
Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis and its host plant is gymnosperms, especially for masson pine
Functional annotation of the C. pinicolalis antennal unigenes In total, 98,214 unigenes were successfully annotated in all databases (Table 2), including 47,089 (47.94%) unigenes matched to known proteins and 33,852 unigenes (34.46%) in the Swiss-Prot database
Under the molecular function category, the genes expressed in the antennae were mostly related to binding, catalytic activity and transporter activity (Additional file 1: Figure S1B)
Summary
Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. Honda and Mitsuhashi identified and distinguished the difference between these pests in the adults, larvae and pupal stages [8]; Konno et al reported that they were different species from their response to different spectra of host-plant constituents [9]; In 2006, the pinaceae-feeding type was named as C. pinicolalis [10]. Though these studies have provided important information regarding the identification of species, it is not entirely reliable because these insect groups were undergoing speciation, genomic changes, or evolving into new taxon [11]. Wang et al used mitochondrial DNA sequencing technique to verify C. pinicolalis and C. punctiferalis were significantly different species [15]
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