Abstract
Fast-sedimenting fractions of sexual agglutination activity digested from Hansenula wingei mating type 5 (5-agglutinin) were obtained by centrifuging in guanidine-HCl or in NaCl gradients under conditions designed to produce the highest specific activity. The particles of 5-agglutinin in these fractions ranged from 120 to 300 million molecular weight as determined by light scattering. An average of 1.7 particles of 5-agglutinin per cell was found at the agglutination endpoint. This number is reasonably close to the theoretical limit of 0.5 particle per cell, and is comparable to the value of approximately unity for other precipitating and agglutinating systems.
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