Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique

Carine Novato Ramos,Tácita Borges Barros,Márcio Borba Da Silva,Bernardo Mirabal,Laize Tomazi,Ricardo Evangelista Fraga,Edma Santos De Antonio

doi:10.33448/rsd-v10i12.20601

Abstract

The sexing birds is considered an important tool for behavioral studies and programs for the reintroduction of animals into the wild. Several techniques are used for this purpose, such as laparoscopy, magnetic resonance and molecular sexing. The first are considered more invasive and stressful for the animal, and the last is considered the most accurate. According to it, the aim of this study was to compare the effectiveness of using three sets of primers in the molecular sexing process of true parrots (Amazona aestiva). Blood samples from 10 animals were collected at a Wildlife Screening Center (CETAS) in Bahia, Brazil. The DNA was extracted and the molecular markers amplified by Polymerase Chain Reaction (PCR) using primer pairs P2/P8, 1237L/1272H and 2250F/2718R. The amplified material was visualized with electrophoresis performed at 2% agarose and 12.5% polyacrylamide gels. Among the primer sets used, the 2250F/2718R pair showed the best results for the sexing process, including visualization of the amplified products on an agarose gel. Agarose gel electrophoresis is considered to be faster and cheaper. The results revealed a sample composition of 5 males (0.5) and 5 females (0.5).

Highlights

The biodiversity of birds in South America has a significant prominence
The results obtained through this work were conclusive regarding the molecular sexing of A. aestiva
The results of the present study reveals that the agarose gel presents better performance when used for visualize amplified DNA with Pair of primers 3

Summary

Introduction

The biodiversity of birds in South America has a significant prominence. According to the publication of the BrazilianSociety of Ornithology (2015), much of this emphasis is attributed to Colombia and Brazil, the countries with the greatest biodiversity of birds in the world. The biodiversity of birds in South America has a significant prominence. According to the publication of the Brazilian. Society of Ornithology (2015), much of this emphasis is attributed to Colombia and Brazil, the countries with the greatest biodiversity of birds in the world. Brazil holds the biggest parrot biodiversity, with 87 species described (Piacentini et al., 2015). Brazil stands out for its important wildlife trafficking routes (Renctas, 2016). The Wildlife Screening Centers (CETAS) are involved in the management of wildlife in Brazil. These are units authorized by the Brazilian Institute of the Environment (IBAMA) to receive animals from seizure of traffic, inspection actions and voluntary deliveries (Brasil, 2015).

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Discussion

Conclusion