Sex-Specific Wolbachia Infection Patterns in Populations of Polygraphus proximus Blandford (Coleoptera; Curculionidae: Scolytinae).

Abstract

Simple SummaryWolbachia bacteria are the most common symbionts of insects. These bacteria are ordinarily transmitted via oocyte cytoplasm from mother to progeny, like mitochondria, and are sporadically transmitted from one species to another. The Wolbachia symbionts have evolved to be parasitic (feminization of genetic males, male-killing, parthenogenesis, and cytoplasmic incompatibility) or/and mutualistic (increasing lifespan and fecundity, providing vitamins and nutrients, defending against viruses and parasites). Here we have studied Wolbachia infection in populations of four-eyed fir bark beetle Polygraphus proximus, which is one of the most dangerous pests of Siberian fir forests. A high rate of the only wProx Wolbachia strain in P. proximus populations was found in a vastly studied territory. Surprisingly, females were more often harboring Wolbachia than males. Besides, a comparison of the Wolbachia density in individuals has revealed that females contain much more Wolbachia symbionts than males. We suppose that the difference in infection status, as well as the difference in Wolbachia load between males and females within a population, can be found in some other Wolbachia–host associations.Wolbachia symbionts are maternally inherited bacteria that are widely distributed among Arthropoda hosts. Wolbachia influence their host biology in diverse ways. They may induce reproductive abnormalities, protect hosts against pathogens and parasites, or benefit hosts through metabolic provisioning. The progeny of an infected female are ordinarily infected with Wolbachia; however, Wolbachia have no future in male host progeny because they cannot transmit the symbiont to the next generation. Here, we analyze native and invasive populations of the four-eyed fir bark beetle (Polygraphus proximus) for Wolbachia prevalence and symbiont genetic diversity. This species is a dangerous pest of Siberian fir (Abies sibirica) forests. The native range of P. proximus includes the territories of the Russian Far East, Japan, Korea, and Northeast China, whereas its invasive range includes West Siberia, with further expansion westward. Surprisingly, we revealed a difference in the patterns of Wolbachia prevalence for males and females. Infection rate and Wolbachia titers were higher in females than in males. ST-533, the only haplotype of Wolbachia supergroup B, was associated with a minimum of three out of the five described mitochondrial haplotypes.