Sex-related alterations of aminopyrine metabolism by standardised extract ofEurycoma Longifolia(TAF-273)

Abstract

Previous study showed that the standardised 50% aqueous-methanol extract of Eurycoma longifolia (TAF-273) affected phase 1 aminopyrine metabolism especially in adult male and female rat hepatocytes. The present study evaluated the molecular mechanism of TAF-273 on phase 1 aminopyrine metabolism in adult male and female rat hepatocytes by using ten cellular stimulants/inhibitors. The study suggested that the effect of TAF-273 on hepatic phase 1 aminopyrine metabolism in adult male rats was probably mediated through the inhibition of tyrosine kinases and through the activation of G-protein, protein kinase G, protein kinase A in the cAMP pathway, calmodulin and protein kinase C. On the contrary, the extract affected adult female rat hepatocytes, only through the activation of G-protein, protein kinase G, protein kinase A in the cAMP pathway and protein kinase C. The effect of TAF-273 on aminopyrine metabolism in the presence of trifluoperazine, phorbol myristate acetate and genistein also exhibited significa...