Abstract
This paper reports a modification of the chromodomain helicase DNA binding protein (CHD) method for sex identification in the Japanese Crested Ibis, Nipponia nippon. In this modification, we employed a restriction enzyme, MboII, instead of HaeIII that was used in the original method. The use of MboII gave similar results to those found with HaeIII. The reliability of the modified method was confirmed by applying both methods to adult ibises of known sex and also by sequencing amplified nucleotides from adult birds and chicks. We used the modified method to sex three chicks obtained from a pair of Japanese Crested Ibises from China that was presented by the Chinese government. In addition, we have shown that the embryonic tissue, mainly blood vessels, left in the eggshell after hatch can be a good source of DNA for sexing.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of the Yamashina Institute for Ornithology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
