Abstract
Sex identification in birds is necessary for studying their ecology, behavior and population dynamics and structure. This study was carried out to determine the sex of Nipponia nippon by a Polymerase Chain Reaction (PCR) method. The sexing universal primers of non-ratite birds were employed to amplify the sexing related genes and identify the sex of each individual. A 657 bp CHD gene fragments on Z chromosome in both sexes and a 463 bp female specific CHD fragments on W chromosome were cloned and sequenced. The sequences of these two sexing related genes fragments were similar with CHD-W of Phalacrocorax capillatuss and CHD-Z of Platalea minor individually. This study is valuable for managing and conserving endangered birds.
Highlights
Nipponia nippon, known as the Asian Crested Ibis, is the only member of the genus Nipponia and one of the world's rarest birds (Hirschfeld et al, 2013)
Accurate sex determination is the primary step in captive breeding and valuable for studying N. nippon’s ecology, behavior, population dynamics and structure (Ramos et al, 2009)
Most of the traditional methods of sex identification are not efficient. Those based on Polymerase Chain Reaction (PCR), are feasible for sex identification in birds
Summary
Known as the Asian Crested Ibis, is the only member of the genus Nipponia and one of the world's rarest birds (Hirschfeld et al, 2013). This species was widespread in the Russian Far East, China, Korea and Japan (Li and Li, 1998). Habitat loss and persecution in last century brought this endangered species to the brink of extinction (Yang et al, 2012) It has disappeared from most of its former range, except in Shaanxi province of China, but it has been reintroduced into Japan in 2008 (Nagata and Yamagish, 2013). Several molecular methods have been developed for identifying the sex of sexually monomorphic birds (Dubiec and Zagalska-Neubauer 2006)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: American Journal of Biochemistry and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
