Sex Differences in Mate Preference Between Two Hybridizing Species of Poeciliid Fish

Abstract

When hybridization between two species in secondary contact is costly, natural selection should favor pre-mating isolation barriers. The invasive Gambusia affinis has been introduced to habitats of a closely related species, the endangered Gambusia nobilis. Although other Gambusia species readily hybridize in secondary contact, previous studies in this system found low abundance of hybrids in sympatry. To examine whether hybridization is limited by behavioral pre-mating isolation that may have evolved in allopatry, I examined each species’ mating preferences using individuals from allopatric populations in male and female visual/olfactory association preference tests as well as open mating tests with and without male–male competition. Gambusia affinis and G. nobilis males had significant association preference for conspecific females in visual/olfactory tests. Only G. nobilis females had statistically significant preference for conspecific males. In open mating tests, males of both species had lower chase times overall when in competition, but there was no difference in number of copulation attempts. Males of both species had higher copulatory success rates with conspecific females when in competition, suggesting females may exert some control over copulation success of males. These results suggest that there are differences in mating preferences between these species. This mate choice may act as a pre-mating isolating barrier to reduce hybridization in sympatry, a proposed threat to the endangered G. nobilis.